Journal: International Journal of Molecular Sciences
Article Title: Effect of Tryptophan-Derived AhR Ligands, Kynurenine, Kynurenic Acid and FICZ, on Proliferation, Cell Cycle Regulation and Cell Death of Melanoma Cells—In Vitro Studies
doi: 10.3390/ijms21217946
Figure Lengend Snippet: The effect of L-KYN on the protein level and cellular localization of cyclin D1 ( a ) and CDK4 ( b ) in melanoma A375 cells. Immunofluorescent staining of cyclin D1 ( a ) and CDK4 ( b ) in A375 cells treated with L-KYN 5 mM for 24 h (control; not treated). Cell nuclei were labeled with cell permeable fluorescent DNA dye DraQ5. Magnification 40×.
Article Snippet: Cell nuclei were labeled with cell permeable fluorescent DNA dye DraQ5 (Cell Signaling Technology, Danvers, MA, USA).
Techniques: Staining, Control, Labeling